TSC1
Gene Ontology Biological Process
- activation of Rho GTPase activity [ISO]
- cardiac muscle cell differentiation [IMP]
- cell projection organization [IMP]
- cell-matrix adhesion [ISO]
- cerebral cortex development [IMP]
- embryo development [IMP]
- glucose import [IMP]
- hippocampus development [IMP]
- kidney development [IMP]
- myelination [IMP]
- negative regulation of TOR signaling [IBA, ISO]
- negative regulation of cell proliferation [IMP, ISO]
- negative regulation of cell size [IGI, IMP]
- negative regulation of insulin receptor signaling pathway [IBA]
- negative regulation of translation [ISO]
- nervous system development [IMP]
- neural tube closure [IMP]
- positive regulation of focal adhesion assembly [ISO]
- potassium ion transport [IGI, IMP]
- protein heterooligomerization [ISO]
- protein stabilization [ISO]
- rRNA export from nucleus [ISO]
- regulation of Ras GTPase activity [IBA]
- regulation of actin cytoskeleton organization [IBA, ISO]
- regulation of cell cycle [IBA]
- regulation of cell-matrix adhesion [ISO]
- regulation of focal adhesion assembly [ISO]
- regulation of phosphoprotein phosphatase activity [ISO]
- regulation of protein kinase activity [IGI]
- regulation of stress fiber assembly [ISO]
- regulation of translation [ISO]
- response to insulin [ISO]
- synapse organization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TSC2
Gene Ontology Biological Process
- acute-phase response [ISO]
- cell projection organization [ISO]
- cellular response to insulin stimulus [IMP]
- establishment of protein localization [ISO]
- glucose import [IMP]
- heart development [IMP]
- insulin-like growth factor receptor signaling pathway [IGI, IMP]
- negative regulation of MAP kinase activity [ISO]
- negative regulation of TOR signaling [IMP, ISO]
- negative regulation of Wnt signaling pathway [IBA, ISO]
- negative regulation of cell proliferation [IGI, IMP, ISO]
- negative regulation of cell size [ISO]
- negative regulation of epithelial cell proliferation [ISO]
- negative regulation of insulin receptor signaling pathway [IBA]
- negative regulation of phosphatidylinositol 3-kinase signaling [IMP]
- negative regulation of protein kinase B signaling [IGI, IMP]
- negative regulation of protein kinase activity [IMP]
- neural tube closure [IMP]
- phosphatidylinositol 3-kinase signaling [IMP]
- positive chemotaxis [IMP]
- positive regulation of GTPase activity [ISO]
- positive regulation of Ras GTPase activity [IBA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein heterooligomerization [ISO]
- protein homooligomerization [ISO]
- protein import into nucleus [IMP]
- protein kinase B signaling [IGI, IMP]
- protein localization [IDA]
- protein transport into plasma membrane raft [IMP]
- regulation of cell cycle [IBA, ISO]
- regulation of endocytosis [IMP]
- regulation of insulin receptor signaling pathway [IMP]
- response to hypoxia [IMP]
- signal transduction [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- TSC1-TSC2 complex [IBA, ISO]
- caveola [ISO]
- cell [IMP]
- cytoplasm [IDA, ISO]
- cytoskeleton [ISO]
- cytosol [ISO]
- dendrite [ISO]
- growth cone [ISO]
- intracellular [IGI]
- intracellular membrane-bounded organelle [ISO]
- membrane [ISO]
- membrane raft [ISO]
- neuron projection [ISO]
- neuronal cell body [ISO]
- perinuclear region of cytoplasm [ISO]
- protein complex [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Tuberin phosphorylation regulates its interaction with hamartin. Two proteins involved in tuberous sclerosis.
Hamartin and tuberin are products of the tumor suppressor genes, TSC1 and TSC2, respectively. When mutated, a characteristic spectrum of tumor-like growths develop resulting in the syndrome of tuberous sclerosis complex. The phenotypes associated with TSC1 and TSC2 mutations are largely indistinguishable suggesting a common biochemical pathway. Indeed, hamartin and tuberin have been shown to interact stably in vitro and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TSC1 TSC2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 2343960 | |
| TSC1 TSC2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TSC2 TSC1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TSC1 TSC2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TSC1 TSC2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID