BAIT
FKBP1A
FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE, RP11-314N13.2
FK506 binding protein 1A, 12kDa
GO Process (26)
GO Function (11)
GO Component (7)
Gene Ontology Biological Process
- 'de novo' protein folding [TAS]
- SMAD protein complex assembly [IDA]
- T cell activation [NAS]
- amyloid fibril formation [IDA]
- calcium ion transmembrane transport [NAS]
- chaperone-mediated protein folding [IBA]
- extracellular fibril organization [IDA]
- heart morphogenesis [ISS]
- heart trabecula formation [ISS]
- negative regulation of protein phosphatase type 2B activity [IDA]
- negative regulation of release of sequestered calcium ion into cytosol [IDA]
- negative regulation of ryanodine-sensitive calcium-release channel activity [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- positive regulation of protein binding [IDA]
- positive regulation of protein ubiquitination [IDA]
- protein folding [NAS]
- protein maturation by protein folding [TAS]
- protein peptidyl-prolyl isomerization [IDA]
- protein refolding [TAS]
- regulation of activin receptor signaling pathway [IDA]
- regulation of amyloid precursor protein catabolic process [IGI]
- regulation of immune response [IMP]
- regulation of protein localization [IGI]
- regulation of ryanodine-sensitive calcium-release channel activity [IDA, ISS]
- transforming growth factor beta receptor signaling pathway [TAS]
- ventricular cardiac muscle tissue morphogenesis [ISS]
Gene Ontology Molecular Function- FK506 binding [IDA, NAS]
- SMAD binding [IPI]
- activin binding [IPI]
- calcium channel inhibitor activity [IDA]
- ion channel binding [ISS, TAS]
- macrolide binding [NAS]
- peptidyl-prolyl cis-trans isomerase activity [IDA, TAS]
- protein binding [IPI]
- signal transducer activity [IMP]
- transforming growth factor beta receptor binding [ISS, TAS]
- type I transforming growth factor beta receptor binding [ISS]
- FK506 binding [IDA, NAS]
- SMAD binding [IPI]
- activin binding [IPI]
- calcium channel inhibitor activity [IDA]
- ion channel binding [ISS, TAS]
- macrolide binding [NAS]
- peptidyl-prolyl cis-trans isomerase activity [IDA, TAS]
- protein binding [IPI]
- signal transducer activity [IMP]
- transforming growth factor beta receptor binding [ISS, TAS]
- type I transforming growth factor beta receptor binding [ISS]
Gene Ontology Cellular Component
Homo sapiens
PREY
MTOR
Frap1, RAFT1
mechanistic target of rapamycin (serine/threonine kinase)
GO Process (40)
GO Function (11)
GO Component (13)
Gene Ontology Biological Process
- TOR signaling [ISO]
- cell growth [ISO]
- cell projection organization [IGI, ISO]
- cellular response to hypoxia [ISO, ISS]
- cellular response to nutrient levels [ISO, ISS]
- double-strand break repair via homologous recombination [IBA]
- germ cell development [ISO]
- negative regulation of NFAT protein import into nucleus [ISO]
- negative regulation of autophagy [ISO, ISS]
- negative regulation of cell size [IMP, ISO]
- negative regulation of macroautophagy [ISO]
- peptidyl-serine phosphorylation [ISO]
- peptidyl-threonine phosphorylation [ISO]
- phosphorylation [ISO]
- positive regulation of actin filament polymerization [ISO]
- positive regulation of endothelial cell proliferation [IMP]
- positive regulation of gene expression [ISO]
- positive regulation of lamellipodium assembly [ISO]
- positive regulation of lipid biosynthetic process [ISO, ISS]
- positive regulation of myotube differentiation [ISO]
- positive regulation of peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of stress fiber assembly [ISO]
- positive regulation of transcription from RNA polymerase III promoter [ISO]
- positive regulation of translation [IMP, ISO]
- protein autophosphorylation [IMP, ISO]
- protein phosphorylation [IDA, IMP, ISO]
- regulation of Rac GTPase activity [ISO]
- regulation of actin cytoskeleton organization [ISO]
- regulation of carbohydrate metabolic process [IDA, IMP]
- regulation of carbohydrate utilization [IDA, IMP]
- regulation of fatty acid beta-oxidation [IDA, IMP]
- regulation of glycogen biosynthetic process [IDA, IMP]
- regulation of myelination [ISO]
- regulation of protein kinase activity [ISO]
- regulation of response to food [IDA, IMP]
- response to amino acid [ISO]
- response to insulin [ISO]
- ruffle organization [ISO]
Gene Ontology Molecular Function- RNA polymerase III type 1 promoter DNA binding [ISO]
- RNA polymerase III type 2 promoter DNA binding [ISO]
- RNA polymerase III type 3 promoter DNA binding [ISO]
- TFIIIC-class transcription factor binding [ISO]
- kinase activity [ISO]
- phosphoprotein binding [ISO]
- protein binding [IPI]
- protein dimerization activity [IBA]
- protein domain specific binding [IPI]
- protein serine/threonine kinase activity [IDA, IMP, ISO]
- ribosome binding [ISO]
- RNA polymerase III type 1 promoter DNA binding [ISO]
- RNA polymerase III type 2 promoter DNA binding [ISO]
- RNA polymerase III type 3 promoter DNA binding [ISO]
- TFIIIC-class transcription factor binding [ISO]
- kinase activity [ISO]
- phosphoprotein binding [ISO]
- protein binding [IPI]
- protein dimerization activity [IBA]
- protein domain specific binding [IPI]
- protein serine/threonine kinase activity [IDA, IMP, ISO]
- ribosome binding [ISO]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Differential effects of rapamycin on mammalian target of rapamycin signaling functions in mammalian cells.
Rapamycin and its analogues have shown promising anticancer activities in preclinical and clinical studies. However, the mechanism whereby rapamycin inhibits signaling through the mammalian target of rapamycin (mTOR) remains poorly understood. Here, we show that the FKBP12/rapamycin complex is an essentially irreversible inhibitor of mTOR kinase activity in vitro. However, we observe no suppression of mTOR catalytic activity after immunoprecipitation ... [more]
Cancer Res. Dec. 01, 2003; 63(23);8451-60 [Pubmed: 14679009]
Throughput
- Low Throughput
Curated By
- BioGRID