An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Low density lipoprotein receptor-related protein and factor IXa share structural requirements for binding to the A3 domain of coagulation factor VIII.

Bovenschen N, Boertjes RC, van Stempvoort G, Voorberg J, Lenting PJ, Meijer AB, Mertens K

Low-density lipoprotein receptor-related protein (LRP) is an endocytic receptor that binds multiple distinct ligands, including blood coagulation factor VIII (FVIII). FVIII is a heterodimeric multidomain protein that consists of a heavy chain (domains A1, a1, A2, a2, and B) and a light chain (domains a3, A3, C1, and C2). Both chains contribute to high-affinity interaction with LRP. One LRP-interactive region ... [more]

J. Biol. Chem. Mar. 14, 2003; 278(11);9370-7 [Pubmed: 12522143]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

LRP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

apolipoprotein binding [IDA, IPI]calcium ion binding [ISS]lipoprotein particle receptor binding [IC]lipoprotein transporter activity [NAS]poly(A) RNA binding [IDA]protein binding [IPI]protein complex binding [IDA]receptor activity [TAS]

Gene Ontology Cellular Component

F8