BAIT

LTB4R2

BLT2, BLTR2, JULF2, KPG_004, LTB4-R 2, LTB4-R2, NOP9

leukotriene B4 receptor 2

Arachidonic Acid Pathway Project

GO Process (2)

GO Function (1)

GO Component (3)

Gene Ontology Biological Process

chemotaxis [TAS]

negative regulation of adenylate cyclase activity [TAS]

Gene Ontology Molecular Function

leukotriene receptor activity [TAS]

Gene Ontology Cellular Component

integral component of plasma membrane [TAS]

plasma membrane [TAS]

CRISPR Database OMIM HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PREY

PER1

PER, RIGUI, hPER

period circadian clock 1

GO Process (20)

GO Function (6)

GO Component (1)

Gene Ontology Biological Process

circadian regulation of gene expression [IDA]

circadian regulation of translation [ISS]

circadian rhythm [IEP]

entrainment of circadian clock [TAS]

entrainment of circadian clock by photoperiod [ISS]

histone H3 acetylation [IDA]

histone H3 deacetylation [ISS]

histone H4 acetylation [IDA]

negative regulation of I-kappaB kinase/NF-kappaB signaling [ISS]

negative regulation of JNK cascade [ISS]

negative regulation of glucocorticoid receptor signaling pathway [ISS]

negative regulation of transcription from RNA polymerase II promoter [ISS]

negative regulation of transcription, DNA-templated [ISS]

positive regulation of transcription from RNA polymerase II promoter [ISS]

posttranscriptional regulation of gene expression [ISS]

regulation of circadian rhythm [ISS]

regulation of cytokine production involved in inflammatory response [ISS]

regulation of hair cycle [IMP]

regulation of p38MAPK cascade [ISS]

regulation of sodium ion transport [ISS]

Gene Ontology Molecular Function

E-box binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
chromatin DNA binding [ISS]
kinase binding [IPI]
transcription factor binding transcription factor activity [ISS]
ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

cytoplasm [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.

Sokolina K, Kittanakom S, Snider J, Kotlyar M, Maurice P, Gandia J, Benleulmi-Chaachoua A, Tadagaki K, Oishi A, Wong V, Malty RH, Deineko V, Aoki H, Amin S, Yao Z, Morato X, Otasek D, Kobayashi H, Menendez J, Auerbach D, Angers S, Przulj N, Bouvier M, Babu M, Ciruela F, Jockers R, Jurisica I, Stagljar I

G-protein-coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex biochemical features. To obtain a global view of GPCR-mediated signaling and to identify novel components of their pathways, we used a modified membrane yeast two-hybrid (MYTH) ... [more]

Mol. Syst. Biol. Dec. 15, 2016; 13(3);918 [Pubmed: 28298427]

Throughput

High Throughput

Additional Notes

interaction identified using a modified split-ubiquitin membrane yeast two-hybrid (MYTH) assay

Curated By

BioGRID