BAIT
PTGER4
EP4, EP4R
prostaglandin E receptor 4 (subtype EP4)
GO Process (15)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
- ERK1 and ERK2 cascade [ISS]
- JNK cascade [ISS]
- T-helper cell differentiation [ISS]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [IDA]
- bone development [ISS]
- cellular response to mechanical stimulus [ISS]
- immune response [IEP]
- negative regulation of cytokine secretion [ISS]
- negative regulation of eosinophil extravasation [IDA]
- negative regulation of inflammatory response [IDA]
- negative regulation of integrin activation [IDA]
- positive regulation of cytokine secretion [ISS]
- positive regulation of inflammatory response [ISS]
- regulation of stress fiber assembly [ISS]
- response to mechanical stimulus [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
KLC1
KLC, KNS2, KNS2A, RP11-73M18.7
kinesin light chain 1
GO Process (5)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [ISS, TAS]
- growth cone [ISS]
- kinesin complex [ISS]
- membrane [IDA]
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
G-protein-coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex biochemical features. To obtain a global view of GPCR-mediated signaling and to identify novel components of their pathways, we used a modified membrane yeast two-hybrid (MYTH) ... [more]
Mol. Syst. Biol. Dec. 15, 2016; 13(3);918 [Pubmed: 28298427]
Throughput
- High Throughput
Additional Notes
- interaction identified using a modified split-ubiquitin membrane yeast two-hybrid (MYTH) assay
Curated By
- BioGRID