An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis.

Capalbo L, Bassi ZI, Geymonat M, Todesca S, Copoiu L, Enright AJ, Callaini G, Riparbelli MG, Yu L, Choudhary JS, Ferrero E, Wheatley S, Douglas ME, Mishima M, D'Avino PP

The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue organization, and cilium and lumen formation. Here, we report the characterization of the intricate midbody protein-protein interaction network (interactome), which identifies many ... [more]

Unknown Dec. 04, 2018; 10(1);4513 [Pubmed: 31586073]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

KIF20A

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IBA]microtubule motor activity [IBA]protein binding [IPI]protein kinase binding [IPI]transporter activity [TAS]

Gene Ontology Cellular Component

AHNAK

Gene Ontology Biological Process

Gene Ontology Molecular Function

S100 protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]structural molecule activity conferring elasticity [ISS]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis.

Throughput

Curated By

ATPase activity [IBA]
microtubule motor activity [IBA]
protein binding [IPI]
protein kinase binding [IPI]
transporter activity [TAS]

S100 protein binding [IPI]
poly(A) RNA binding [IDA]
protein binding [IPI]
structural molecule activity conferring elasticity [ISS]