An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Deubiquitinase USP28 inhibits ubiquitin ligase KLHL2-mediated uridine-cytidine kinase 1 degradation and confers sensitivity to 5'-azacytidine-resistant human leukemia cells.

Zhang H, Huang H, Feng X, Song H, Zhang Z, Shen A, Qiu X

Resistance to the chemotherapeutic drug 5'-azacytidine (5'-AZA) is a major obstacle in the treatment of patients with acute myeloid leukemia (AML). The uridine-cytidine kinase 1 (UCK1) has an established role in activating 5'-AZA and its protein level is significantly downregulated in patients resistant to the drug. However, the underlying molecular mechanism for the reduced UCK1 expression remains to be elucidated. ... [more]

Theranostics Jan. 16, 2020; 10(3);1046-1059 [Pubmed: 31938050]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

UCK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

nucleoside kinase activity [EXP]

Gene Ontology Cellular Component

PGK1