BAIT

VRK-1

CELE_F28B12.3, tag-223, F28B12.3
vrk-1 encodes the ortholog of the vaccinia-related protein kinases in metazoans; vrk-1 is required for normal mitosis, proper nuclear envelope formation and regulates the association of the LEM domain protein BAF-1/Barrier-to-autointegration factor (and possibly other LEM domain proteins) with chromatin via BAF-1 phosphorylation; vrk-1 is required for germ cell proliferation and acts in part through regulation of CEP-1/p53; vrk-1 is also required for development of the egg-laying system including several processes like formation of the vulva, the specification and proliferation of uterine cells and sex myoblasts, and anchor cell invasion of the basement membrane between the vulva and somatic gonad; VRK-1 is expressed in neurons, hypodermal cells and vulval cells, and localizes to the nuclear envelope and chromatin in a cell-cylce dependent manner.
Caenorhabditis elegans
PREY

BAF-1

CELE_B0464.7, pna-1, 3J557, B0464.7
baf-1 encodes a small, novel protein that is highly conserved amongst metazoa; BAF-1 binds double-stranded DNA nonspecifically in vitro and is essential for proper chromosome segregation, embryogenesis, and gonad development in vivo; biochemical and phenotypic analyses suggest that BAF-1 functions as an intramolecular bridge that mediates nuclear assembly and chromatin capture by the reforming nuclear envelope; by homology with mammalian BAF proteins, BAF-1 is also predicted to play a role in regulating gene expression and higher-order chromatin structure; during interphase, BAF-1 colocalizes with LMN-1/lamin to the nuclear envelope and nuclear interior; during mitosis, BAF-1 is present in a more punctate pattern, localizing near condensing chromatin and maintaining this localization through telophase; BAF-1 localization during nuclear assembly requires the activity of LMN-1/lamin, EMR-1/emerin, and LEM-2/MAN1; baf-1 mutations also exhibit hypersensitivity to DNA damage and baf-1 exhibits genetics interactions with lem-3, suggesting that these two genes function together to regulate the DNA damage response.
Caenorhabditis elegans

Biochemical Activity

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Caenorhabditis elegans BAF-1 and its kinase VRK-1 participate directly in post-mitotic nuclear envelope assembly.

Gorjanacz M, Klerkx EP, Galy V, Santarella R, Lopez-Iglesias C, Askjaer P, Mattaj IW

Barrier-to-autointegration factor (BAF) is an essential, highly conserved, metazoan protein. BAF interacts with LEM (LAP2, emerin, MAN1) domain-carrying proteins of the inner nuclear membrane. We analyzed the in vivo function of BAF in Caenorhabditis elegans embryos using both RNA interference and a temperature-sensitive baf-1 gene mutation and found that BAF is directly involved in nuclear envelope (NE) formation. NE defects ... [more]

EMBO J Jan. 10, 2007; 26(1);132-43 [Pubmed: 17170708]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
VRK-1 BAF-1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-WormBase
-
VRK-1 BAF-1
Biochemical Activity
Biochemical Activity

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Low-WormBase
2835800

Curated By