LET-60
Gene Ontology Biological Process
- body morphogenesis [IMP]
- embryo development ending in birth or egg hatching [IMP]
- epidermal growth factor receptor signaling pathway [IGI]
- hermaphrodite genitalia development [IMP]
- locomotion [IMP]
- muscle organ development [IGI, IMP]
- negative regulation of vulval development [IMP]
- nematode larval development [IGI, IMP]
- oogenesis [IGI]
- positive regulation of Rap GTPase activity [IDA]
- positive regulation of Ras GTPase activity [IDA]
- positive regulation of vulval development [IGI, IMP]
- receptor-mediated endocytosis [IMP]
- regulation of cell fate specification [IMP]
- regulation of cell projection organization [IGI, IMP]
- reproduction [IGI, IMP]
- secretion by cell [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PLC-1
Gene Ontology Biological Process
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Identification of PLC210, a Caenorhabditis elegans phospholipase C, as a putative effector of Ras.
Mammalian Ras proteins regulate multiple effectors including Raf, Ral guanine nucleotide dissociation stimulator (RalGDS), and phosphoinositide 3-kinase. In the nematode Caenorhabditis elegans, LIN-45 Raf has been identified by genetic analyses as an effector of LET-60 Ras. To search for other effectors in C. elegans, we performed a yeast two-hybrid screening for LET-60-binding proteins. The screening identified two cDNA clones encoding ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
LET-60 PLC-1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | 3.9583 | BioGRID | 466433 |