BAIT
ACS2
acetate--CoA ligase ACS2, L000003111, YLR153C
Acetyl-coA synthetase isoform; along with Acs1p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
GO Process (3)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
VPS15
GRD8, VAC4, VPL19, VPS40, VPT15, ubiquitin-binding serine/threonine protein kinase VPS15, L000002470, L000002924, S000029641, L000002480, YBR097W
Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
GO Process (9)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- inositol lipid-mediated signaling [IMP]
- late endosome to vacuole transport [IMP]
- macroautophagy [IMP]
- peroxisome degradation [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- protein phosphorylation [IDA]
- protein retention in Golgi apparatus [IMP]
- protein targeting to vacuole [IMP]
- vacuole inheritance [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
A comprehensive synthetic genetic interaction network governing yeast histone acetylation and deacetylation.
Histone acetylation and deacetylation are among the principal mechanisms by which chromatin is regulated during transcription, DNA silencing, and DNA repair. We analyzed patterns of genetic interactions uncovered during comprehensive genome-wide analyses in yeast to probe how histone acetyltransferase (HAT) and histone deacetylase (HDAC) protein complexes interact. The genetic interaction data unveil an underappreciated role of HDACs in maintaining cellular ... [more]
Genes Dev. Aug. 01, 2008; 22(15);2062-74 [Pubmed: 18676811]
Throughput
- High Throughput|Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- High Throughput: dSLAM analysis was performed to determine genome-wide genetic interaction profiles of 38 query genes involved in histone (de)acetylation.
- Low Throughput: Genetic interactions identified using dSLAM were validated by tetrad dissection and/or random spore analysis.
Curated By
- BioGRID