BAIT
ARHGAP31
AOS1, CDGAP
Rho GTPase activating protein 31
GO Process (2)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Homo sapiens
PREY
LIMD1
LIM domains containing 1
GO Process (16)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- cell migration [IMP]
- cytoplasmic mRNA processing body assembly [IMP]
- cytoskeleton organization [IMP]
- gene silencing by miRNA [IMP]
- multicellular organismal development [TAS]
- negative regulation of canonical Wnt signaling pathway [ISS]
- negative regulation of hippo signaling [IDA]
- negative regulation of osteoblast differentiation [ISS]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- osteoblast development [ISS]
- phosphorylation [IDA]
- positive regulation of gene silencing by miRNA [IMP]
- regulation of cell shape [IMP]
- regulation of transcription, DNA-templated [TAS]
- response to hypoxia [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions.
Rho GTPases are central regulators of the cytoskeleton and, in humans, are controlled by 145 multidomain guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs). How Rho signalling patterns are established in dynamic cell spaces to control cellular morphogenesis is unclear. Through a family-wide characterization of substrate specificities, interactomes and localization, we reveal at the systems level how RhoGEFs and ... [more]
Nat. Cell Biol. Dec. 01, 2019; 22(4);498-511 [Pubmed: 32203420]
Quantitative Score
- 4.151067068 [CompPASS Z-score]
Throughput
- High Throughput
Additional Notes
- prey proteins were classified into confidence-level categories of gold (highest confidence), silver or bronze based on various criteria
- tagged bait proteins were affinity purified and associated proteins identified by mass spectrometry
- the score provided is the CompPASS Z-score
- this interaction was classified by the authors as being in the silver list
Curated By
- BioGRID