DHX30
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EIF2AK2
Gene Ontology Biological Process
- activation of MAPKK activity [IMP]
- evasion or tolerance by virus of host immune response [TAS]
- modulation by virus of host morphology or physiology [TAS]
- modulation by virus of host process [TAS]
- negative regulation of cell proliferation [TAS]
- negative regulation of osteoblast proliferation [IMP]
- negative regulation of translation [IDA, IMP]
- negative regulation of viral genome replication [IMP]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of NIK/NF-kappaB signaling [ISS]
- positive regulation of chemokine production [ISS]
- positive regulation of cytokine production [ISS]
- positive regulation of stress-activated MAPK cascade [ISS]
- protein autophosphorylation [IDA, IMP]
- protein phosphorylation [IDA]
- regulation of NLRP3 inflammasome complex assembly [ISS]
- regulation of hematopoietic progenitor cell differentiation [ISS]
- regulation of hematopoietic stem cell differentiation [ISS]
- regulation of hematopoietic stem cell proliferation [ISS]
- response to interferon-alpha [IDA]
- response to virus [IMP]
- viral life cycle [TAS]
Gene Ontology Molecular Function
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A High-Density Human Mitochondrial Proximity Interaction Network.
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously annotated as mitochondrial, nearly half of the mitochondrial proteome defined by Mitocarta 2.0. Bait-bait analysis showed a clear separation of ... [more]
Quantitative Score
- 1.0 [Saint Score]
Throughput
- High Throughput
Additional Notes
- interaction assayed using BioID
- interactions were considered high confidence if they had a Bayesian False Discovery Rate of 1% or less
- the Saint Score for the interaction (or the maximum of any bait-prey combinations that had multiple scores) is shown
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EIF2AK2 DHX30 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1224 | BioGRID | 3481480 | |
| EIF2AK2 DHX30 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 594161 | |
| EIF2AK2 DHX30 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 941080 | |
| EIF2AK2 DHX30 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID