BAIT
SLC25A46
solute carrier family 25, member 46
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
PLK1
PLK, STPK13
polo-like kinase 1
GO Process (34)
GO Function (7)
GO Component (14)
Gene Ontology Biological Process
- G2 DNA damage checkpoint [IDA]
- G2/M transition of mitotic cell cycle [IDA, TAS]
- activation of mitotic anaphase-promoting complex activity [IDA]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- cell proliferation [TAS]
- centrosome organization [IMP]
- cytokinesis [IDA, IMP]
- establishment of protein localization [IMP]
- metaphase/anaphase transition of mitotic cell cycle [TAS]
- microtubule bundle formation [IDA]
- mitotic cell cycle [TAS]
- mitotic cytokinesis [IDA]
- mitotic nuclear division [IDA, IMP]
- mitotic nuclear envelope disassembly [TAS]
- mitotic sister chromatid segregation [IMP]
- mitotic spindle assembly checkpoint [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- peptidyl-serine phosphorylation [IDA]
- positive regulation of peptidyl-threonine phosphorylation [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of proteolysis [IDA]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- positive regulation of ubiquitin-protein transferase activity [IMP]
- protein destabilization [IDA]
- protein localization to chromatin [IDA]
- protein phosphorylation [IDA]
- protein ubiquitination [IDA]
- regulation of cell cycle [TAS]
- regulation of mitotic cell cycle [IMP]
- regulation of mitotic metaphase/anaphase transition [IMP]
- regulation of protein binding [IMP]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A High-Density Human Mitochondrial Proximity Interaction Network.
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously annotated as mitochondrial, nearly half of the mitochondrial proteome defined by Mitocarta 2.0. Bait-bait analysis showed a clear separation of ... [more]
Cell Metab. Sep. 01, 2020; 32(3);479-497.e9 [Pubmed: 32877691]
Quantitative Score
- 1.0 [Saint Score]
Throughput
- High Throughput
Additional Notes
- interaction assayed using BioID
- interactions were considered high confidence if they had a Bayesian False Discovery Rate of 1% or less
- the Saint Score for the interaction (or the maximum of any bait-prey combinations that had multiple scores) is shown
Curated By
- BioGRID