Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Network-Based Combinatorial CRISPR-Cas9 Screens Identify Synergistic Modules in Human Cells.

Guo Y, Bao C, Ma D, Cao Y, Li Y, Xie Z, Li S

Tumorigenesis is a complex process that is driven by a combination of networks of genes and environmental factors; however, efficient approaches to identifying functional networks that are perturbed by the process of tumorigenesis are lacking. In this study, we provide a comprehensive network-based strategy for the systematic discovery of functional synergistic modules that are causal determinants of inflammation-induced tumorigenesis. Our ... [more]

ACS Synth Biol Dec. 15, 2018; 8(3);482-490 [Pubmed: 30762338]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: viability (PATO:0000169)
  • phenotype: growth abnormality (HP:0001507)

Additional Notes

  • CRISPR GI screen
  • Cell Line:NCM460 cells
  • Experimental Setup: Cytokine Exposure (TGFB1)
  • GIST: A-phenotypic positive genetic interaction
  • Library: Targeted Library
  • Significance Threshold: dGI > 1.11

Curated By

  • BioGRID