PF4
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IBA]
- blood coagulation [TAS]
- chemokine-mediated signaling pathway [IBA]
- cytokine-mediated signaling pathway [IDA]
- immune response [IBA]
- inflammatory response [IBA]
- leukocyte chemotaxis [IDA]
- negative regulation of MHC class II biosynthetic process [IDA]
- negative regulation of angiogenesis [IDA]
- negative regulation of cytolysis [IDA]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [IDA]
- negative regulation of megakaryocyte differentiation [IDA]
- platelet activation [IDA, TAS]
- platelet degranulation [TAS]
- positive regulation of cAMP metabolic process [IDA]
- positive regulation of cAMP-mediated signaling [IDA]
- positive regulation of gene expression [IDA]
- positive regulation of leukocyte chemotaxis [IBA]
- positive regulation of macrophage derived foam cell differentiation [IDA]
- positive regulation of macrophage differentiation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of tumor necrosis factor production [IDA]
- regulation of cell proliferation [IDA]
- response to lipopolysaccharide [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CXCL8
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- angiogenesis [TAS]
- calcium-mediated signaling [TAS]
- cell cycle arrest [IDA]
- cellular component movement [TAS]
- cellular protein metabolic process [TAS]
- cellular response to fibroblast growth factor stimulus [IEP]
- cellular response to interleukin-1 [IEP]
- cellular response to lipopolysaccharide [IDA]
- cellular response to tumor necrosis factor [IEP]
- chemokine-mediated signaling pathway [IBA]
- chemotaxis [TAS]
- embryonic digestive tract development [IEP]
- endoplasmic reticulum unfolded protein response [TAS]
- immune response [IBA]
- induction of positive chemotaxis [IGI]
- inflammatory response [TAS]
- intracellular signal transduction [IDA]
- negative regulation of G-protein coupled receptor protein signaling pathway [IDA]
- negative regulation of cell proliferation [TAS]
- neutrophil activation [TAS]
- neutrophil chemotaxis [IGI]
- positive regulation of neutrophil chemotaxis [TAS]
- receptor internalization [IDA]
- regulation of cell adhesion [IDA]
- regulation of single stranded viral RNA replication via double stranded DNA intermediate [IDA]
- response to endoplasmic reticulum stress [IDA]
- response to molecule of bacterial origin [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.
Chemokines orchestrate leukocyte trafficking and function in health and disease. Heterophilic interactions between chemokines in a given microenvironment may amplify, inhibit, or modulate their activity; however, a systematic evaluation of the chemokine interactome has not been performed. We used immunoligand blotting and surface plasmon resonance to obtain a comprehensive map of chemokine-chemokine interactions and to confirm their specificity. Structure-function analyses ... [more]
Quantitative Score
- 0.248 [Relative OD]
Throughput
- High Throughput
Additional Notes
- averages of 2-4 experiments were used
- interaction assayed using densitometry analysis of bidirectional immunoligand blotting in which the bait protein was immobilized on a membrane, the prey protein was added in solution, prey was detected by specific antibody, and the densitometric optical density (OD) was normalized for area and reported as a ratio of a corresponding positive control
- interactions were considered positive if this relative ratio was equal to or greater than 0.05
Curated By
- BioGRID