BAIT

PPBP

B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1
pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
Homo sapiens
PREY

CCL28

CCK1, MEC, SCYA28
chemokine (C-C motif) ligand 28
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.

von Hundelshausen P, Agten SM, Eckardt V, Blanchet X, Schmitt MM, Ippel H, Neideck C, Bidzhekov K, Leberzammer J, Wichapong K, Faussner A, Drechsler M, Grommes J, van Geffen JP, Li H, Ortega-Gomez A, Megens RT, Naumann R, Dijkgraaf I, Nicolaes GA, Doering Y, Soehnlein O, Lutgens E, Heemskerk JW, Koenen RR, Mayo KH, Hackeng TM, Weber C

Chemokines orchestrate leukocyte trafficking and function in health and disease. Heterophilic interactions between chemokines in a given microenvironment may amplify, inhibit, or modulate their activity; however, a systematic evaluation of the chemokine interactome has not been performed. We used immunoligand blotting and surface plasmon resonance to obtain a comprehensive map of chemokine-chemokine interactions and to confirm their specificity. Structure-function analyses ... [more]

Sci Transl Med Dec. 05, 2016; 9(384); [Pubmed: 28381538]

Quantitative Score

  • 0.211 [Relative OD]

Throughput

  • High Throughput

Additional Notes

  • averages of 2-4 experiments were used
  • interaction assayed using densitometry analysis of bidirectional immunoligand blotting in which the bait protein was immobilized on a membrane, the prey protein was added in solution, prey was detected by specific antibody, and the densitometric optical density (OD) was normalized for area and reported as a ratio of a corresponding positive control
  • interactions were considered positive if this relative ratio was equal to or greater than 0.05

Curated By

  • BioGRID