BAIT
FASN
FAS, OA-519, SDR27X1
fatty acid synthase
GO Process (11)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- energy reserve metabolic process [TAS]
- fatty acid metabolic process [TAS]
- long-chain fatty-acyl-CoA biosynthetic process [TAS]
- osteoblast differentiation [IDA]
- pantothenate metabolic process [TAS]
- positive regulation of cellular metabolic process [TAS]
- small molecule metabolic process [TAS]
- triglyceride biosynthetic process [TAS]
- vitamin metabolic process [TAS]
- water-soluble vitamin metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ALOX12
12-LOX, 12S-LOX, LOG12
arachidonate 12-lipoxygenase
GO Process (23)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- arachidonic acid metabolic process [IDA, ISS, TAS]
- cellular component movement [IMP]
- establishment of skin barrier [ISS]
- fatty acid oxidation [IMP]
- hepoxilin biosynthetic process [ISS]
- hepoxilin metabolic process [TAS]
- leukotriene A4 metabolic process [IDA]
- linoleic acid metabolic process [IDA]
- lipoxin A4 biosynthetic process [IDA]
- lipoxin B4 biosynthetic process [IDA]
- lipoxin metabolic process [TAS]
- lipoxygenase pathway [IDA, TAS]
- negative regulation of apoptotic process [IMP, ISS]
- negative regulation of muscle cell apoptotic process [IMP]
- negative regulation of platelet aggregation [ISS]
- positive regulation of angiogenesis [IMP]
- positive regulation of cell adhesion [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IMP, ISS]
- reactive oxygen species metabolic process [NAS]
- small molecule metabolic process [TAS]
- superoxide anion generation [NAS]
Gene Ontology Molecular Function- arachidonate 12-lipoxygenase activity [IDA, TAS]
- hepoxilin A3 synthase activity [TAS]
- hepoxilin-epoxide hydrolase activity [ISS]
- linoleate 13S-lipoxygenase activity [IDA]
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen [TAS]
- protein binding [IPI]
- arachidonate 12-lipoxygenase activity [IDA, TAS]
- hepoxilin A3 synthase activity [TAS]
- hepoxilin-epoxide hydrolase activity [ISS]
- linoleate 13S-lipoxygenase activity [IDA]
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen [TAS]
- protein binding [IPI]
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.
The de novo synthesis of fatty acids has emerged as a therapeutic target for various diseases, including cancer. Because cancer cells are intrinsically buffered to combat metabolic stress, it is important to understand how cells may adapt to the loss of de novo fatty acid biosynthesis. Here, we use pooled genome-wide CRISPR screens to systematically map genetic interactions (GIs) in ... [more]
Nat Metab Jun. 01, 2020; 2(6);499-513 [Pubmed: 32694731]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507)
- viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: HAP1
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: TKOv3
- Significance Threshold: -0.5>qGI>0.5; false-discovery rate (FDR)<0.5
Curated By
- BioGRID