SD
Gene Ontology Biological Process
- cardiac muscle cell development [IMP]
- compound eye morphogenesis [IMP]
- epithelium development [IMP]
- germarium-derived female germ-line cyst formation [IMP]
- imaginal disc-derived leg morphogenesis [IMP]
- imaginal disc-derived wing morphogenesis [IDA, IMP, TAS]
- negative regulation of hippo signaling [IGI]
- nervous system development [NAS]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP]
- regulation of transcription from RNA polymerase II promoter [ISS]
- sensory organ development [IMP]
- somatic muscle development [IMP]
- stem cell proliferation [IDA]
- wing disc development [IGI, IMP]
Gene Ontology Molecular Function- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
NERFIN-1
Gene Ontology Biological Process
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transcription factor networks in Drosophila melanogaster.
Specific cellular fates and functions depend on differential gene expression, which occurs primarily at the transcriptional level and is controlled by complex regulatory networks of transcription factors (TFs). TFs act through combinatorial interactions with other TFs, cofactors, and chromatin-remodeling proteins. Here, we define protein-protein interactions using a coaffinity purification/mass spectrometry method and study 459 Drosophila melanogaster transcription-related factors, representing approximately ... [more]
Quantitative Score
- 35.400413 [HGSCore]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified using a HyperGeometric Spectral Counts scoring method (HGSCore) and were filtered to a false discovery rate (FDR) of 2%.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NERFIN-1 SD | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| SD NERFIN-1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| NERFIN-1 SD | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID