SD
Gene Ontology Biological Process
- cardiac muscle cell development [IMP]
- compound eye morphogenesis [IMP]
- epithelium development [IMP]
- germarium-derived female germ-line cyst formation [IMP]
- imaginal disc-derived leg morphogenesis [IMP]
- imaginal disc-derived wing morphogenesis [IDA, IMP, TAS]
- negative regulation of hippo signaling [IGI]
- nervous system development [NAS]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP]
- regulation of transcription from RNA polymerase II promoter [ISS]
- sensory organ development [IMP]
- somatic muscle development [IMP]
- stem cell proliferation [IDA]
- wing disc development [IGI, IMP]
Gene Ontology Molecular Function- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
NERFIN-1
Gene Ontology Biological Process
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The Scalloped and Nerfin-1 Transcription Factors Cooperate to Maintain Neuronal Cell Fate.
The ability of cells to stably maintain their fate is governed by specific transcription regulators. Here, we show that the Scalloped (Sd) and Nervous fingers-1 (Nerfin-1) transcription factors physically and functionally interact to maintain medulla neuron fate in the Drosophila melanogaster CNS. Using Targeted DamID, we find that Sd and Nerfin-1 occupy a highly overlapping set of target genes, including ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SD NERFIN-1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 35.4004 | BioGRID | 2866542 | |
| NERFIN-1 SD | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| NERFIN-1 SD | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |