BOWL
Gene Ontology Biological Process
- embryonic foregut morphogenesis [IMP]
- embryonic hindgut morphogenesis [IMP]
- hindgut morphogenesis [IMP]
- imaginal disc-derived leg joint morphogenesis [IMP]
- imaginal disc-derived leg morphogenesis [IMP]
- lateral inhibition [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- negative regulation of transcription, DNA-templated [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of transcription, DNA-templated [ISS]
- terminal region determination [IMP]
- wing disc development [IMP]
Gene Ontology Molecular Function
LIN
Gene Ontology Biological Process
- embryonic foregut morphogenesis [IMP]
- embryonic hindgut morphogenesis [IMP]
- embryonic pattern specification [IMP]
- epidermis development [IMP]
- foregut morphogenesis [IMP]
- hindgut morphogenesis [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- proximal/distal axis specification [IMP]
- spiracle morphogenesis, open tracheal system [IMP]
- terminal region determination [IGI]
- torso signaling pathway [IGI]
- wing disc development [IMP]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The Drumstick/Lines/Bowl regulatory pathway links antagonistic Hedgehog and Wingless signaling inputs to epidermal cell differentiation.
Hedgehog and Wingless signaling in the Drosophila embryonic epidermis represents one paradigm for organizer function. In patterning this epidermis, Hedgehog and Wingless act asymmetrically, and consequently otherwise equivalent cells on either side of the organizer follow distinct developmental fates. To better understand the downstream mechanisms involved, we have investigated mutations that disrupt dorsal epidermal pattern. We have previously demonstrated that ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| LIN BOWL | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| BOWL LIN | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |