Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

The SCAN domain of ZNF174 is a dimer.

Stone JR, Maki JL, Blacklow SC, Collins T

The SCAN domain is a conserved region of 84 residues found predominantly in zinc finger DNA-binding proteins in vertebrates. The SCAN domain appears to control the association of SCAN domain containing proteins into noncovalent complexes and may be the primary mechanism underlying partner choice in the oligomerization of these transcription factors. Here we have overexpressed, purified, and characterized the isolated ... [more]

J. Biol. Chem. Feb. 15, 2002; 277(7);5448-52 [Pubmed: 11741982]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ZNF174 ZNF174
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID