SINAH
Gene Ontology Biological Process
Gene Ontology Molecular Function
TTK
Gene Ontology Biological Process
- R1/R6 development [IMP]
- R7 cell development [IMP, TAS]
- border follicle cell migration [IMP]
- brain morphogenesis [IMP]
- branch fusion, open tracheal system [IMP]
- branching involved in open tracheal system development [IMP]
- cell fate determination [TAS]
- chitin-based cuticle development [IMP]
- compound eye cone cell differentiation [IMP]
- compound eye corneal lens morphogenesis [IMP]
- dendrite morphogenesis [IMP]
- dorsal appendage formation [IMP]
- dorsal trunk growth, open tracheal system [IMP]
- imaginal disc-derived wing morphogenesis [IMP]
- inter-male aggressive behavior [IMP]
- locomotion involved in locomotory behavior [IMP]
- negative regulation of transcription from RNA polymerase II promoter [NAS]
- negative regulation of transcription, DNA-templated [IDA, IGI]
- neuron development [IMP]
- ovarian follicle cell development [IMP]
- peripheral nervous system development [TAS]
- positive regulation of DNA binding [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of cell shape [IMP]
- regulation of compound eye cone cell fate specification [IGI, IMP]
- regulation of embryonic cell shape [IMP]
- regulation of transcription from RNA polymerase II promoter [NAS]
- regulation of tube size, open tracheal system [IMP]
- startle response [IMP]
- tracheal outgrowth, open tracheal system [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- POZ domain binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA, IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
- DNA binding [IDA]
- POZ domain binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA, IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Two modes of degradation of the tramtrack transcription factors by Siah homologues.
The Siah proteins, mammalian homologues of the Drosophila Sina protein, function as ubiquitin-protein isopeptide ligase enzymes to target a wide range of cellular proteins for degradation. We report here a novel Drosophila protein that is homologous to Sina, named Sina-Homologue (SinaH). We show that it can direct the degradation of the transcriptional repressor Tramtrack (Ttk) using two different mechanisms. One ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TTK SINAH | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| SINAH TTK | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |