SINAH
Gene Ontology Biological Process
Gene Ontology Molecular Function
TTK
Gene Ontology Biological Process
- R1/R6 development [IMP]
- R7 cell development [IMP, TAS]
- border follicle cell migration [IMP]
- brain morphogenesis [IMP]
- branch fusion, open tracheal system [IMP]
- branching involved in open tracheal system development [IMP]
- cell fate determination [TAS]
- chitin-based cuticle development [IMP]
- compound eye cone cell differentiation [IMP]
- compound eye corneal lens morphogenesis [IMP]
- dendrite morphogenesis [IMP]
- dorsal appendage formation [IMP]
- dorsal trunk growth, open tracheal system [IMP]
- imaginal disc-derived wing morphogenesis [IMP]
- inter-male aggressive behavior [IMP]
- locomotion involved in locomotory behavior [IMP]
- negative regulation of transcription from RNA polymerase II promoter [NAS]
- negative regulation of transcription, DNA-templated [IDA, IGI]
- neuron development [IMP]
- ovarian follicle cell development [IMP]
- peripheral nervous system development [TAS]
- positive regulation of DNA binding [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of cell shape [IMP]
- regulation of compound eye cone cell fate specification [IGI, IMP]
- regulation of embryonic cell shape [IMP]
- regulation of transcription from RNA polymerase II promoter [NAS]
- regulation of tube size, open tracheal system [IMP]
- startle response [IMP]
- tracheal outgrowth, open tracheal system [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- POZ domain binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA, IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
- DNA binding [IDA]
- POZ domain binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA, IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Next-generation large-scale binary protein interaction network for Drosophila melanogaster.
Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of?>?10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of ... [more]
Throughput
- High Throughput
Additional Notes
- interaction identified by large-scale Y2H screening and validated by pairwise testing
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SINAH TTK | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| TTK SINAH | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| SINAH TTK | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID