BAIT

HMG-3

CELE_C32F10.5, C32F10.5
HMG
Caenorhabditis elegans
PREY

F19F10.9

CELE_F19F10.9
The F19F10.9 gene encodes a homolog of the human SART1 gene, which encodes a protein recognised by IgE that may be involved in atopic disease.
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

FACT Sets a Barrier for Cell Fate Reprogramming in Caenorhabditis elegans and Human Cells.

Kolundzic E, Ofenbauer A, Bulut SI, Uyar B, Baytek G, Sommermeier A, Seelk S, He M, Hirsekorn A, Vucicevic D, Akalin A, Diecke S, Lacadie SA, Tursun B

The chromatin regulator FACT (facilitates chromatin transcription) is essential for ensuring stable gene expression by promoting transcription. In a genetic screen using Caenorhabditis elegans, we identified that FACT maintains cell identities and acts as a barrier for transcription factor-mediated cell fate reprogramming. Strikingly, FACT's role as a barrier to cell fate conversion is conserved in humans as we show that ... [more]

Dev Cell Dec. 10, 2017; 46(5);611-626.e12 [Pubmed: 30078731]

Throughput

  • High Throughput

Additional Notes

  • bait proteins were affinity purified and associated prey proteins were identified by mass spectrometry
  • score is p-value

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HMG-3 F19F10.9
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3704168

Curated By

  • BioGRID