BAIT

HMG-3

CELE_C32F10.5, C32F10.5
HMG
Caenorhabditis elegans
PREY

F19F10.9

CELE_F19F10.9
The F19F10.9 gene encodes a homolog of the human SART1 gene, which encodes a protein recognised by IgE that may be involved in atopic disease.
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Robust co-immunoprecipitation with mass spectrometry for Caenorhabditis elegans using solid-phase enhanced sample preparation.

Baytek G, Popp O, Mertins P, Tursun B

Studying protein interactions in vivo can reveal key molecular mechanisms of biological processes. Co-immunoprecipitation with mass spectrometry detects protein-protein interactions with high throughput. The nematode Caenorhabditis elegans is a powerful genetic model organism for in vivo studies. Yet its rigid and complex tissues require optimization for biochemistry applications to ensure reproducibility. The authors optimized co-immunoprecipitation with mass spectrometry by combining ... [more]

Biotechniques May. 01, 2022; 72(5);175-184 [Pubmed: 35297663]

Throughput

  • High Throughput

Additional Notes

  • interaction seen in the presence of DNAse

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HMG-3 F19F10.9
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2875145

Curated By

  • BioGRID