ATG5
Gene Ontology Biological Process
- C-terminal protein lipidation [IBA]
- autophagic vacuole assembly [IMP]
- autophagy [IMP]
- blood vessel remodeling [IMP]
- cellular response to nitrogen starvation [IBA]
- heart contraction [IMP]
- mitochondrion degradation [IBA]
- negative regulation of apoptotic process [IMP]
- negative regulation of histone H4-K16 acetylation [IMP]
- negative regulation of protein ubiquitination [IMP]
- negative stranded viral RNA replication [IMP]
- nucleophagy [IBA]
- otolith development [IMP]
- positive regulation of mucus secretion [IMP]
- post-translational protein modification [IDA]
- regulation of cilium assembly [IMP]
- regulation of cytokine secretion involved in immune response [IMP]
- regulation of reactive oxygen species metabolic process [IMP]
- regulation of release of sequestered calcium ion into cytosol [IMP]
- response to drug [IMP]
- response to fungus [IMP]
- vasodilation [IMP]
- ventricular cardiac muscle cell development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CASP8
Gene Ontology Biological Process
- TRAIL-activated apoptotic signaling pathway [ISO]
- activation of cysteine-type endopeptidase activity [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- angiogenesis [IMP]
- apoptotic process [IDA, ISO]
- apoptotic signaling pathway [IDA, ISO]
- cardiac muscle tissue development [TAS]
- execution phase of apoptosis [ISO]
- extrinsic apoptotic signaling pathway [IDA, IGI, ISO]
- extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- heart development [IMP]
- hepatocyte apoptotic process [IMP]
- macrophage differentiation [IMP, TAS]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- negative regulation of necroptotic process [IGI]
- neural tube formation [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of extrinsic apoptotic signaling pathway [IGI]
- positive regulation of macrophage differentiation [ISO]
- positive regulation of proteolysis [ISO]
- protein heterooligomerization [ISO]
- proteolysis [IGI, ISO]
- proteolysis involved in cellular protein catabolic process [ISO]
- regulation of apoptotic signaling pathway [IGI]
- regulation of thymocyte apoptotic process [IGI]
- response to ethanol [ISO]
- response to tumor necrosis factor [ISO]
Gene Ontology Molecular Function- cysteine-type endopeptidase activity [IDA, IGI, ISO]
- cysteine-type endopeptidase activity involved in apoptotic process [IDA, ISO]
- cysteine-type endopeptidase activity involved in apoptotic signaling pathway [ISO]
- death effector domain binding [IBA, ISO]
- death receptor binding [ISO]
- endopeptidase activity [IDA, IMP]
- peptidase activity [IDA, IMP, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [IPI]
- scaffold protein binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- cysteine-type endopeptidase activity [IDA, IGI, ISO]
- cysteine-type endopeptidase activity involved in apoptotic process [IDA, ISO]
- cysteine-type endopeptidase activity involved in apoptotic signaling pathway [ISO]
- death effector domain binding [IBA, ISO]
- death receptor binding [ISO]
- endopeptidase activity [IDA, IMP]
- peptidase activity [IDA, IMP, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [IPI]
- scaffold protein binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- CD95 death-inducing signaling complex [ISO]
- Noc1p-Noc2p complex [IMP]
- cell body [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- death-inducing signaling complex [ISO]
- membrane raft [ISO]
- microtubule organizing center [ISO]
- mitochondrion [IDA, ISO]
- neuron projection [ISO]
- nucleoplasm [ISO]
- nucleus [IDA]
- protein complex [ISO]
- ripoptosome [ISO]
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Autophagy genes in myeloid cells counteract IFN?-induced TNF-mediated cell death and fatal TNF-induced shock.
Host inflammatory responses must be tightly regulated to ensure effective immunity while limiting tissue injury. IFN gamma (IFN?) primes macrophages to mount robust inflammatory responses. However, IFN? also induces cell death, and the pathways that regulate IFN?-induced cell death are incompletely understood. Using genome-wide CRISPR/Cas9 screening, we identified autophagy genes as central mediators of myeloid cell survival during the IFN? ... [more]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen: Increased ligand resistance
- Cell Line: BV-2 (BTO:0003350) Atg5 knockout (Atg5KO) cells
- Experimental Setup: Ligand Exposure (Interferon gamma)
- Library: Brie (mouse)
- Significance Threshold: Positive STARS score FDR < 0.1
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATG5 CASP8 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ATG5 CASP8 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID