Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Autophagy genes in myeloid cells counteract IFN?-induced TNF-mediated cell death and fatal TNF-induced shock.

Orvedahl A, McAllaster MR, Sansone A, Dunlap BF, Desai C, Wang YT, Balce DR, Luke CJ, Lee S, Orchard RC, Artyomov MN, Handley SA, Doench JG, Silverman GA, Virgin HW

Host inflammatory responses must be tightly regulated to ensure effective immunity while limiting tissue injury. IFN gamma (IFN?) primes macrophages to mount robust inflammatory responses. However, IFN? also induces cell death, and the pathways that regulate IFN?-induced cell death are incompletely understood. Using genome-wide CRISPR/Cas9 screening, we identified autophagy genes as central mediators of myeloid cell survival during the IFN? ... [more]

Proc Natl Acad Sci U S A Dec. 13, 2018; 116(33);16497-16506 [Pubmed: 31346084]

Throughput

High Throughput

Additional Notes

CRISPR GI screen: Increased ligand resistance
Cell Line: BV-2 (BTO:0003350) Atg5 knockout (Atg5KO) cells
Experimental Setup: Ligand Exposure (Interferon gamma)
Library: Brie (mouse)
Significance Threshold: Positive STARS score FDR < 0.1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ATG5 FADD	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Bell BD (2008)	Low	-	BioGRID	-
FADD ATG5	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Bell BD (2008)	Low	-	BioGRID	-
ATG5 FADD	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Young MM (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ATG5

Gene Ontology Biological Process

Gene Ontology Molecular Function

Atg8 ligase activity [IBA]protein binding [IPI]

Gene Ontology Cellular Component

FADD

Gene Ontology Biological Process

Gene Ontology Molecular Function

death effector domain binding [ISO]death receptor binding [ISO]identical protein binding [ISO]protease binding [ISO]protein binding [IPI]protein complex binding [ISO]tumor necrosis factor receptor binding [ISO]tumor necrosis factor receptor superfamily binding [ISO]