BAIT

INS

IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
insulin
GO Process (60)
GO Function (6)
GO Component (6)

Gene Ontology Biological Process

Homo sapiens
PREY

SLC25A22

EIEE3, GC1, NET44
solute carrier family 25 (mitochondrial carrier: glutamate), member 22
GO Process (2)
GO Function (1)
GO Component (3)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role for Peroxiredoxin 4 in Proinsulin Folding.

Tran DT, Pottekat A, Mir SA, Loguercio S, Jang I, Campos AR, Scully KM, Lahmy R, Liu M, Arvan P, Balch WE, Kaufman RJ, Itkin-Ansari P

The ?-cell protein synthetic machinery is dedicated to the production of mature insulin, which requires the proper folding and trafficking of its precursor, proinsulin. The complete network of proteins that mediate proinsulin folding and advancement through the secretory pathway, however, remains poorly defined. Here we used affinity purification and mass spectrometry to identify, for the first time, the proinsulin biosynthetic ... [more]

Diabetes Aug. 01, 2020; 69(8);1723-1734 [Pubmed: 32457219]

Throughput

  • High Throughput

Curated By

  • BioGRID