An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Wang C, Li Y, Yan S, Wang H, Shao X, Xiao M, Yang B, Qin G, Kong R, Chen R, Zhang N

Interacting with proteins is a crucial way for long noncoding RNAs (lncRNAs) to exert their biological responses. Here we report a high throughput strategy to characterize lncRNA interacting proteins in vivo by combining tobramycin affinity purification and mass spectrometric analysis (TOBAP-MS). Using this method, we identify 140 candidate binding proteins for lncRNA highly upregulated in liver cancer (HULC). Intriguingly, HULC ... [more]

Nat Commun Dec. 22, 2019; 11(1);3162 [Pubmed: 32572027]

Throughput

High Throughput

Additional Notes

RAP-MS and Tobramycin affinity purification of lncRNA-HULC

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LDHA HULC	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Wang C (2020)	Low	-	BioGRID	-
HULC LDHA	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Wang C (2020)	Low	-	BioGRID	-
LDHA HULC	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Wang C (2020)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

HULC

LDHA

Gene Ontology Biological Process

Gene Ontology Molecular Function

L-lactate dehydrogenase activity [NAS]protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS