BAIT

HULC

HCCAT1, LINC00078, NCRNA00078

hepatocellular carcinoma up-regulated long non-coding RNA

GO Process (0)

GO Function (0)

GO Component (0)

CRISPR Database OMIM HGNC Alliance of Genome Resources Entrez Gene Ensembl

Homo sapiens

PREY

OXR1

TLDC3, Nbla00307

oxidation resistance 1

GO Process (1)

GO Function (0)

GO Component (0)

Gene Ontology Biological Process

response to oxidative stress [NAS]

CRISPR Database OMIM HGNC Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Wang C, Li Y, Yan S, Wang H, Shao X, Xiao M, Yang B, Qin G, Kong R, Chen R, Zhang N

Interacting with proteins is a crucial way for long noncoding RNAs (lncRNAs) to exert their biological responses. Here we report a high throughput strategy to characterize lncRNA interacting proteins in vivo by combining tobramycin affinity purification and mass spectrometric analysis (TOBAP-MS). Using this method, we identify 140 candidate binding proteins for lncRNA highly upregulated in liver cancer (HULC). Intriguingly, HULC ... [more]

Nat Commun Dec. 22, 2019; 11(1);3162 [Pubmed: 32572027]

Throughput

High Throughput

Additional Notes

Tobramycin affinity purification of lncRNA-HULC

Curated By

BioGRID

Interaction Summary