BAIT

ALOX12

12-LOX, 12S-LOX, LOG12
arachidonate 12-lipoxygenase
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening.

Tang K, Finley RL, Nie D, Honn KV

The platelet isoform of 12-lipoxygenase (12-LOX) is expressed in a variety of human tumors. 12-LOX metabolizes arachidonic acid to 12(S)-hydroxyeicosateraenoic acid (12(S)-HETE), which induces a number of cellular responses associated with tumor progression and metastasis. Little is known about 12-LOX regulation and no direct regulators of 12-LOX activity have been identified. To identify potential regulators of 12-LOX, we isolated cDNAs ... [more]

Biochemistry Mar. 28, 2000; 39(12);3185-91 [Pubmed: 10727209]

Throughput

  • Low Throughput

Curated By

  • BioGRID