ZAP70
Gene Ontology Biological Process
- B cell activation [TAS]
- T cell activation [TAS]
- T cell aggregation [TAS]
- T cell differentiation [NAS]
- T cell migration [TAS]
- T cell receptor signaling pathway [IBA, TAS]
- adaptive immune response [TAS]
- cell migration [IBA]
- immune response [IDA]
- innate immune response [IBA]
- intracellular signal transduction [NAS]
- peptidyl-tyrosine autophosphorylation [IBA]
- peptidyl-tyrosine phosphorylation [IDA, IMP]
- positive regulation of T cell differentiation [IDA]
- positive regulation of alpha-beta T cell proliferation [IBA]
- positive thymic T cell selection [IDA]
- protein phosphorylation [IDA, NAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PLCG1
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- T cell receptor signaling pathway [TAS]
- activation of MAPKK activity [TAS]
- activation of phospholipase C activity [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- calcium-mediated signaling [IMP]
- cell migration [IMP]
- cellular response to epidermal growth factor stimulus [IDA, IMP]
- cytokine-mediated signaling pathway [TAS]
- epidermal growth factor receptor signaling pathway [IMP, TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- inositol phosphate metabolic process [TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of angiogenesis [IDA]
- positive regulation of blood vessel endothelial cell migration [IDA]
- positive regulation of epithelial cell migration [IMP]
- positive regulation of release of sequestered calcium ion into cytosol [IMP]
- signal transduction [NAS, TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ligation of the T-cell antigen receptor (TCR) induces association of hSos1, ZAP-70, phospholipase C-gamma 1, and other phosphoproteins with Grb2 and the zeta-chain of the TCR.
Signaling by the T-cell antigen receptor (TCR) involves both phospholipase C (PLC)-gamma 1 and p21ras activation. While failing to induce Shc/Grb2 association, ligation of the TCR/CD3 receptor in Jurkat T-cells induced hSos1-Grb2 complexes. In addition to hSos1, Grb2 participates in the formation of a tyrosine phosphoprotein complex that includes 145-, 95-, 70-, 54-, and 36-38-kDa proteins. p145 was identified as ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PLCG1 ZAP70 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| ZAP70 PLCG1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID