EGLN2
Gene Ontology Biological Process
- cell redox homeostasis [IDA]
- cellular response to hypoxia [TAS]
- intracellular estrogen receptor signaling pathway [NAS]
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline [IDA]
- positive regulation of protein catabolic process [IDA]
- regulation of cell growth [NAS]
- regulation of neuron apoptotic process [IMP]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- response to hypoxia [IDA]
Gene Ontology Molecular Function- ferrous iron binding [NAS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- oxygen sensor activity [IDA]
- peptidyl-proline 4-dioxygenase activity [IDA]
- protein binding [IPI]
- ferrous iron binding [NAS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- oxygen sensor activity [IDA]
- peptidyl-proline 4-dioxygenase activity [IDA]
- protein binding [IPI]
Gene Ontology Cellular Component
- nucleoplasm [IDA, TAS]
- nucleus [IDA]
BRD4
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IMP]
- chromatin remodeling [IDA]
- negative regulation of DNA damage checkpoint [IMP]
- positive regulation of G2/M transition of mitotic cell cycle [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of inflammatory response [IDA]
- regulation of phosphorylation of RNA polymerase II C-terminal domain [IDA]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Targeted and Interactome Proteomics Revealed the Role of PHD2 in Regulating BRD4 Proline Hydroxylation.
Proline hydroxylation is a critical cellular mechanism regulating energy homeostasis and development. Our previous study identified and validated Bromodomain-containing protein 4 (BRD4) as a proline hydroxylation substrate in cancer cells. Yet, the regulatory mechanism and the functional significance of the modification remain unknown. In this study, we developed targeted quantification assays using parallel-reaction monitoring and biochemical analysis to identify the ... [more]
Throughput
- Low Throughput
Additional Notes
- Source of PHD1/EGLN2 not clear
Curated By
- BioGRID