BAIT

PIP1B

ATHH2, F4I18.6, NAMED PLASMA MEMBRANE INTRINSIC PROTEIN 1;2, PIP1;2, TMP-A, TRANSMEMBRANE PROTEIN A, plasma membrane intrinsic protein 1B, AT2G45960
aquaporin PIP1-2
GO Process (5)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

PIP1C

F22L4.16, F22L4_16, PIP1;3, PLASMA MEMBRANE INTRINSIC PROTEIN 1;3, TMP-B, plasma membrane intrinsic protein 1C, AT1G01620
aquaporin PIP1-3
GO Process (3)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Novel Aquaporin Regulatory Mechanisms Revealed by Interactomics.

Bellati J, Champeyroux C, Hem S, Rofidal V, Krouk G, Maurel C, Santoni V

PIP1;2 and PIP2;1 are aquaporins that are highly expressed in roots and bring a major contribution to root water transport and its regulation by hormonal and abiotic factors. Interactions between cellular proteins or with other macromolecules contribute to forming molecular machines. Proteins that molecularly interact with PIP1;2 and PIP2;1 were searched to get new insights into regulatory mechanisms of root ... [more]

Mol Cell Proteomics Dec. 01, 2015; 15(11);3473-3487 [Pubmed: 27609422]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PIP1B PIP1C
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
1253597

Curated By

  • BioGRID