Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Ras-activated endocytosis is mediated by the Rab5 guanine nucleotide exchange activity of RIN1.

Tall GG, Barbieri MA, Stahl PD, Horazdovsky BF

RIN1 was originally identified by its ability to inhibit activated Ras and likely participates in multiple signaling pathways because it binds c-ABL and 14-3-3 proteins, in addition to Ras. RIN1 also contains a region homologous to the catalytic domain of Vps9p-like Rab guanine nucleotide exchange factors (GEFs). Here, we show that this region is necessary and sufficient for RIN1 interaction ... [more]

Dev. Cell Jul. 01, 2001; 1(1);73-82 [Pubmed: 11703925]

Throughput

  • Low Throughput

Curated By

  • BioGRID