BAIT

MPP5

PALS1
membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
GO Process (4)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

E6

HpV16gp1
transforming protein
Human Papilloma Virus (HPV) Project
Synthetic Protein Interaction Project
GO Process (0)
GO Function (0)
GO Component (0)
Human papillomavirus (16)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Quantifying domain-ligand affinities and specificities by high-throughput holdup assay.

Vincentelli R, Luck K, Poirson J, Polanowska J, Abdat J, Blemont M, Turchetto J, Iv F, Ricquier K, Straub ML, Forster A, Cassonnet P, Borg JP, Jacob Y, Masson M, Nomine Y, Reboul J, Wolff N, Charbonnier S, Trave G

Many protein interactions are mediated by small linear motifs interacting specifically with defined families of globular domains. Quantifying the specificity of a motif requires measuring and comparing its binding affinities to all its putative target domains. To this end, we developed the high-throughput holdup assay, a chromatographic approach that can measure up to 1,000 domain-motif equilibrium binding affinities per day. ... [more]

Nat Methods Aug. 01, 2015; 12(8);787-93 [Pubmed: 26053890]

Throughput

  • Low Throughput

Ontology Terms

  • uterine cervix (BTO:0001421)
  • hela cell (BTO:0000567) [cervical adenocarcinoma cell (BTO:0003165)]
  • epithelial cell of cervix (CL:0002535)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
E6 MPP5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3830132
E6 MPP5
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

High-BioGRID
-

Curated By

  • BioGRID