BAIT

E6

HpV16gp1
transforming protein
Human Papilloma Virus (HPV) Project
Synthetic Protein Interaction Project
GO Process (0)
GO Function (0)
GO Component (0)
Human papillomavirus (16)
PREY

MPP5

PALS1
membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
GO Process (4)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The human PDZome: a gateway to PSD95-Disc large-zonula occludens (PDZ)-mediated functions.

Belotti E, Polanowska J, Daulat AM, Audebert S, Thome V, Lissitzky JC, Lembo F, Blibek K, Omi S, Lenfant N, Gangar A, Montcouquiol M, Santoni MJ, Sebbagh M, Aurrand-Lions M, Angers S, Kodjabachian L, Reboul J, Borg JP

Protein-protein interactions organize the localization, clustering, signal transduction, and degradation of cellular proteins and are therefore implicated in numerous biological functions. These interactions are mediated by specialized domains able to bind to modified or unmodified peptides present in binding partners. Among the most broadly distributed protein interaction domains, PSD95-disc large-zonula occludens (PDZ) domains are usually able to bind carboxy-terminal sequences ... [more]

Mol Cell Proteomics Sep. 01, 2013; 12(9);2587-603 [Pubmed: 23722234]

Throughput

  • High Throughput

Ontology Terms

  • hek-293t cell (BTO:0002181)
  • kidney epithelial cell (CL:0002518)
  • kidney (BTO:0000671)
  • caco-2 cell (BTO:0000195) [colonic adenocarcinoma cell (BTO:0000032)]
  • colon epithelial cell (CL:0011108)
  • colon (BTO:0000269)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MPP5 E6
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
2885659
E6 MPP5
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

High-BioGRID
-

Curated By

  • BioGRID