BAIT
NPC1
NPC
Niemann-Pick disease, type C1
GO Process (10)
GO Function (5)
GO Component (10)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ATP1A1
RP4-655J12.1
ATPase, Na+/K+ transporting, alpha 1 polypeptide
GO Process (14)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
- cardiac muscle contraction [TAS]
- cell communication by electrical coupling involved in cardiac conduction [TAS]
- cellular potassium ion homeostasis [IDA]
- cellular response to steroid hormone stimulus [IDA]
- cellular sodium ion homeostasis [IDA]
- ion transmembrane transport [TAS]
- membrane repolarization [IDA]
- membrane repolarization during cardiac muscle cell action potential [IC]
- potassium ion import [IDA]
- regulation of sodium ion transport [ISS]
- relaxation of cardiac muscle [TAS]
- response to glycoside [IDA]
- sodium ion export from cell [IDA]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export.
Cholesterol import in mammalian cells is mediated by the LDL receptor pathway. Here, we perform a genome-wide CRISPR screen using an endogenous cholesterol reporter and identify >100 genes involved in LDL-cholesterol import. We characterise C18orf8 as a core subunit of the mammalian Mon1-Ccz1 guanidine exchange factor (GEF) for Rab7, required for complex stability and function. C18orf8-deficient cells lack Rab7 activation ... [more]
Nat Commun Dec. 03, 2019; 11(1);5559 [Pubmed: 33144569]
Throughput
- High Throughput
Curated By
- BioGRID