BAIT

DCX

DBCN, DC, LISX, SCLH, XLIS, RP5-914P14.1
doublecortin
GO Process (4)
GO Function (3)
GO Component (4)
Homo sapiens
PREY

PAFAH1B1

LIS1, LIS2, MDCR, MDS, PAFAH
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Interaction between LIS1 and doublecortin, two lissencephaly gene products.

Caspi M, Atlas R, Kantor A, Sapir T, Reiner O

Mutations in either LIS1 or DCX are the most common cause for type I lissencephaly. Here we report that LIS1 and DCX interact physically both in vitro and in vivo. Epitope-tagged DCX transiently expressed in COS cells can be co-immunoprecipitated with endogenous LIS1. Furthermore, endogenous DCX could be co-immunoprecipitated with endogenous LIS1 in embryonic brain extracts, demonstrating an in vivo ... [more]

Hum. Mol. Genet. Sep. 22, 2000; 9(15);2205-13 [Pubmed: 11001923]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DCX PAFAH1B1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
PAFAH1B1 DCX
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
PAFAH1B1 DCX
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
PAFAH1B1 DCX
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID