Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Presenilin/?-secretase-dependent EphA3 processing mediates axon elongation through non-muscle myosin IIA.

Javier-Torrent M, Marco S, Rocandio D, Pons-Vizcarra M, Janes PW, Lackmann M, Egea J, Saura CA

EphA/ephrin signaling regulates axon growth and guidance of neurons, but whether this process occurs also independently of ephrins is unclear. We show that presenilin-1 (PS1)/?-secretase is required for axon growth in the developing mouse brain. PS1/?-secretase mediates axon growth by inhibiting RhoA signaling and cleaving EphA3 independently of ligand to generate an intracellular domain (ICD) fragment that reverses axon defects ... [more]

Elife Dec. 02, 2018; 8(); [Pubmed: 31577226]

Throughput

  • Low Throughput

Curated By

  • BioGRID