BAIT

TOP3

EDR1, DNA topoisomerase 3, L000002321, YLR234W
DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity stimulated by RPA and Sgs1p-Top2p-Rmi1p; involved in telomere stability and regulation of mitotic recombination
Saccharomyces cerevisiae (S288c)
PREY

MET5

ECM17, sulfite reductase (NADPH) subunit beta, L000001080, L000003892, YJR137C
Sulfite reductase beta subunit; involved in amino acid biosynthesis, transcription repressed by methionine
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Network Rewiring of Homologous Recombination Enzymes during Mitotic Proliferation and Meiosis.

Wild P, Susperregui A, Piazza I, Doerig C, Oke A, Arter M, Yamaguchi M, Hilditch AT, Vuina K, Chan KC, Gromova T, Haber JE, Fung JC, Picotti P, Matos J

Homologous recombination (HR) is essential for high-fidelity DNA repair during mitotic proliferation and meiosis. Yet, context-specific modifications must tailor the recombination machinery to avoid (mitosis) or enforce (meiosis) the formation of reciprocal exchanges-crossovers-between recombining chromosomes. To obtain molecular insight into how crossover control is achieved, we affinity purified 7 DNA-processing enzymes that channel HR intermediates into crossovers or noncrossovers from ... [more]

Mol Cell Dec. 22, 2018; 75(4);859-874.e4 [Pubmed: 31351878]

Throughput

  • High Throughput

Additional Notes

  • mitotic proliferation
  • saint score >0.9

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MET5 TOP3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2153BioGRID
2140723

Curated By

  • BioGRID