USP1
Gene Ontology Biological Process
- monoubiquitinated protein deubiquitination [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IBA, ISO]
- proteolysis [ISO]
- regulation of DNA repair [IBA, ISO]
- regulation of proteasomal protein catabolic process [IBA]
- response to UV [ISO]
- skeletal system development [IMP]
Gene Ontology Molecular Function
WDR48
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IMP]
- double-strand break repair via homologous recombination [IMP]
- embryonic organ development [IMP]
- homeostasis of number of cells [IMP]
- male gonad development [IMP]
- multicellular organism growth [IMP]
- positive regulation of epithelial cell proliferation [IMP]
- protein deubiquitination [ISO]
- regulation of protein monoubiquitination [IMP]
- seminiferous tubule development [IMP]
- single fertilization [IMP]
- skeletal system morphogenesis [IMP]
- skin development [IMP]
- spermatogenesis [IMP]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation.
PI3K-Akt-FoxO-mTOR signaling is the central pathway controlling growth and metabolism in all cells. Ubiquitination of the protein kinase Akt prior to its phosphorylation is required for PI3K-Akt activity. Here, we found that the deubiquitinating (DUB) enzyme USP1 removes K63-linked polyubiquitin chains on Akt to restrict PI3K-Akt-FoxO signaling in mouse muscle during prolonged starvation. DUB screening platform identified USP1 as a ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
USP1 WDR48 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID