BAIT
TSC1
RP23-362N19.3
tuberous sclerosis 1
GO Process (33)
GO Function (5)
GO Component (12)
Gene Ontology Biological Process
- activation of Rho GTPase activity [ISO]
- cardiac muscle cell differentiation [IMP]
- cell projection organization [IMP]
- cell-matrix adhesion [ISO]
- cerebral cortex development [IMP]
- embryo development [IMP]
- glucose import [IMP]
- hippocampus development [IMP]
- kidney development [IMP]
- myelination [IMP]
- negative regulation of TOR signaling [IBA, ISO]
- negative regulation of cell proliferation [IMP, ISO]
- negative regulation of cell size [IGI, IMP]
- negative regulation of insulin receptor signaling pathway [IBA]
- negative regulation of translation [ISO]
- nervous system development [IMP]
- neural tube closure [IMP]
- positive regulation of focal adhesion assembly [ISO]
- potassium ion transport [IGI, IMP]
- protein heterooligomerization [ISO]
- protein stabilization [ISO]
- rRNA export from nucleus [ISO]
- regulation of Ras GTPase activity [IBA]
- regulation of actin cytoskeleton organization [IBA, ISO]
- regulation of cell cycle [IBA]
- regulation of cell-matrix adhesion [ISO]
- regulation of focal adhesion assembly [ISO]
- regulation of phosphoprotein phosphatase activity [ISO]
- regulation of protein kinase activity [IGI]
- regulation of stress fiber assembly [ISO]
- regulation of translation [ISO]
- response to insulin [ISO]
- synapse organization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
DAB2
5730435J12Rik, AA960054, AI957090, D15Wsu122e, D630005B22Rik, Doc-2, Doc2, p96, mCG_144967
disabled 2, mitogen-responsive phosphoprotein
GO Process (43)
GO Function (10)
GO Component (14)
Gene Ontology Biological Process
- activation of JUN kinase activity [IDA]
- cell morphogenesis involved in differentiation [IMP]
- cellular response to epidermal growth factor stimulus [ISO]
- cellular response to transforming growth factor beta stimulus [IDA]
- clathrin coat assembly [IDA, ISO]
- endoderm development [IMP]
- excretion [IMP]
- in utero embryonic development [IMP]
- leading edge cell differentiation [ISO]
- negative regulation of ERK1 and ERK2 cascade [ISO]
- negative regulation of androgen receptor signaling pathway [ISO]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of canonical Wnt signaling pathway [ISO]
- negative regulation of cell growth [ISO]
- negative regulation of epithelial cell proliferation [ISO]
- negative regulation of extrinsic apoptotic signaling pathway [IMP]
- negative regulation of neuron projection development [ISO]
- negative regulation of protein binding [ISO]
- negative regulation of protein localization to plasma membrane [IMP, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- pinocytosis [IMP]
- positive regulation of SMAD protein import into nucleus [ISO]
- positive regulation of Wnt signaling pathway, planar cell polarity pathway [ISO]
- positive regulation of aldosterone biosynthetic process [ISO]
- positive regulation of aldosterone secretion [ISO]
- positive regulation of cell adhesion [IDA, IMP]
- positive regulation of cell migration [IMP, ISO]
- positive regulation of clathrin-mediated endocytosis [IDA, ISO]
- positive regulation of early endosome to late endosome transport [ISO]
- positive regulation of endocytosis [ISO]
- positive regulation of epithelial to mesenchymal transition [IMP, ISO]
- positive regulation of integrin-mediated signaling pathway [IMP]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IMP, ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of receptor recycling [IMP]
- positive regulation of substrate adhesion-dependent cell spreading [IDA, IMP, ISO]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- positive regulation of transcription, DNA-templated [ISO]
- positive regulation of transforming growth factor beta receptor signaling pathway [ISO]
- receptor-mediated endocytosis [IDA, IPI, ISO]
- regulation of Rho-dependent protein serine/threonine kinase activity [ISO]
- response to steroid hormone [ISO]
Gene Ontology Molecular Function- AP-2 adaptor complex binding [IDA]
- SMAD binding [ISO]
- cargo receptor activity [IDA, ISO]
- clathrin adaptor activity [IDA, ISO]
- clathrin binding [IDA]
- integrin binding [IMP]
- phosphatidylinositol binding [IDA]
- phosphatidylinositol-4,5-bisphosphate binding [IDA]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- AP-2 adaptor complex binding [IDA]
- SMAD binding [ISO]
- cargo receptor activity [IDA, ISO]
- clathrin adaptor activity [IDA, ISO]
- clathrin binding [IDA]
- integrin binding [IMP]
- phosphatidylinositol binding [IDA]
- phosphatidylinositol-4,5-bisphosphate binding [IDA]
- protein C-terminus binding [ISO]
- protein binding [IPI]
Gene Ontology Cellular Component
- apical plasma membrane [IDA]
- clathrin coat of coated pit [IDA]
- clathrin-coated vesicle [ISO]
- clathrin-coated vesicle membrane [ISO]
- coated pit [ISO]
- cytoplasm [IDA]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- intracellular membrane-bounded organelle [ISO]
- nucleolus [ISO]
- nucleus [IDA]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- transforming growth factor beta receptor homodimeric complex [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation.
PI3K-Akt-FoxO-mTOR signaling is the central pathway controlling growth and metabolism in all cells. Ubiquitination of the protein kinase Akt prior to its phosphorylation is required for PI3K-Akt activity. Here, we found that the deubiquitinating (DUB) enzyme USP1 removes K63-linked polyubiquitin chains on Akt to restrict PI3K-Akt-FoxO signaling in mouse muscle during prolonged starvation. DUB screening platform identified USP1 as a ... [more]
EMBO Rep Dec. 03, 2019; 21(4);e48791 [Pubmed: 32133736]
Throughput
- Low Throughput
Curated By
- BioGRID