BAIT

PSF1

CDC101, YDR013W
Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
GO Process (2)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

RAD51

MUT5, recombinase RAD51, L000001571, YER095W
Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Recombination and Pol ? Rescue Defective DNA Replication upon Impaired CMG Helicase-Pol ? Interaction.

Denkiewicz-Kruk M, Jedrychowska M, Endo S, Araki H, Jonczyk P, Dmowski M, Fijalkowska IJ

The CMG complex (Cdc45, Mcm2-7, GINS (Psf1, 2, 3, and Sld5)) is crucial for both DNA replication initiation and fork progression. The CMG helicase interaction with the leading strand DNA polymerase epsilon (Pol ?) is essential for the preferential loading of Pol ? onto the leading strand, the stimulation of the polymerase, and the modulation of helicase activity. Here, we ... [more]

Int J Mol Sci Dec. 13, 2020; 21(24); [Pubmed: 33322195]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: mutation frequency (APO:0000198)
  • phenotype: chromosome/plasmid maintenance (APO:0000143)

Additional Notes

  • genetic complex
  • triple mutants show increased defects in DNA content

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PSF1 RAD51
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
2652828
PSF1 RAD51
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
2652829
RAD51 PSF1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low/High0.0033BioGRID
818582

Curated By

  • BioGRID