BAIT

AP2B1

1300012O03Rik, AI788979, RP23-381B19.6
adaptor-related protein complex 2, beta 1 subunit
GO Process (0)
GO Function (2)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

AMPH

amphiphysin
GO Process (2)
GO Function (5)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

PTEN? regulates endocytosis and modulates olfactory function.

Yuan Y, Zhao X, Wang P, Mei F, Zhou J, Jin Y, McNutt MA, Yin Y

Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) ? is the first identified isoform of the well-known tumor suppressor PTEN. PTEN? has an evolutionarily conserved 173-aa N terminus compared with canonical PTEN. Recently, PTEN? has been shown to play roles in multiple biologic processes including learning and memory, cardiac homeostasis, and antiviral immunity. Here, we report that PTEN? maintains ... [more]

FASEB J Dec. 01, 2018; 33(10);11148-11162 [Pubmed: 31291551]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AMPH AP2B1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID