Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A Case Study on the Keap1 Interaction with Peptide Sequence Epitopes Selected by the Peptidomic mRNA Display.

Hirose H, Hideshima T, Katoh T, Suga H

Many protein-protein and peptide-protein interactions (PPIs) play key roles in the regulation of biological functions, and therefore, the modulation of PPIs has become an attractive target of new drug development. Although a number of PPIs have already been identified, over 100?000 unknown PPIs are predicted to exist. To uncover such unknown PPIs, it is important to devise a conceptually distinct ... [more]

Chembiochem Dec. 16, 2018; 20(16);2089-2100 [Pubmed: 31169361]

Throughput

  • Low Throughput

Additional Notes

  • assayed using SPR (surface plasmon resonance) with peptides derived from the bait proteins immobilized on the SPR membrane resulting in the identification of the hit protein from cell lysates

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KEAP1 ASTN1
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

High-BioGRID
2909631
ASTN1 KEAP1
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Low-BioGRID
2909634

Curated By

  • BioGRID