Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

TNF-? inhibits glucocorticoid receptor-induced gene expression by reshaping the GR nuclear cofactor profile.

Dendoncker K, Timmermans S, Vandewalle J, Eggermont M, Lempiaeinen J, Paakinaho V, Van Hamme E, Dewaele S, Vandevyver S, Ballegeer M, Souffriau J, Van Wyngene L, Van Looveren K, Vanderhaeghen T, Beyaert R, De Bosscher K, Palvimo JJ, Van Montagu M, Libert C

Glucocorticoid resistance (GCR) is defined as an unresponsiveness to the therapeutic effects, including the antiinflammatory ones of glucocorticoids (GCs) and their receptor, the glucocorticoid receptor (GR). It is a problem in the management of inflammatory diseases and can be congenital as well as acquired. The strong proinflammatory cytokine TNF-alpha (TNF) induces an acute form of GCR, not only in mice, ... [more]

Proc Natl Acad Sci U S A Dec. 25, 2018; 116(26);12942-12951 [Pubmed: 31182584]

Throughput

  • High Throughput

Curated By

  • BioGRID