BAIT
DNAJB13
RSPH16A, TSARG5, TSARG6
DnaJ (Hsp40) homolog, subfamily B, member 13
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
AKT2
HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA
v-akt murine thymoma viral oncogene homolog 2
GO Process (19)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- cellular protein modification process [TAS]
- cellular response to insulin stimulus [IMP]
- fat cell differentiation [TAS]
- insulin receptor signaling pathway [IMP, TAS]
- intracellular protein transmembrane transport [ISS]
- mammary gland epithelial cell differentiation [TAS]
- membrane organization [TAS]
- negative regulation of plasma membrane long-chain fatty acid transport [IMP]
- positive regulation of cell motility [IMP]
- positive regulation of fatty acid beta-oxidation [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glucose metabolic process [IMP]
- positive regulation of glycogen biosynthetic process [IMP]
- positive regulation of protein phosphorylation [ISS]
- positive regulation of protein targeting to membrane [ISS]
- positive regulation of vesicle fusion [ISS]
- regulation of cell cycle arrest [TAS]
- regulation of cell migration [TAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell cortex [ISS]
- cytosol [TAS]
- nucleus [IDA, TAS]
- plasma membrane [ISS, TAS]
- ruffle membrane [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains.
Hsp70s comprise a deeply conserved chaperone family that has a central role in maintaining protein homeostasis. In humans, Hsp70 client specificity is provided by 49 different co-factors known as J domain proteins (JDPs). However, the cellular function and client specificity of JDPs have largely remained elusive. We have combined affinity purification-mass spectrometry (AP-MS) and proximity-dependent biotinylation (BioID) to characterize the ... [more]
Mol Cell Apr. 27, 2021; (); [Pubmed: 33957083]
Quantitative Score
- 115.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an average probability of the identified interaction (AvgP) >= 0.98. The associated score represents the fold change of spectral counts (or intensities) for each individual interaction.
Curated By
- BioGRID