An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.

Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Graeslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J

Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]

Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]

Quantitative Score

44.14633481 [HGSCore]

Throughput

High Throughput

Additional Notes

Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PCGF3 PCGF6	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Zhu Y (2022)	Low	-	BioGRID	3502449

Curated By

BioGRID

Interaction Summary

PCGF3

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

PCGF6

Gene Ontology Biological Process