BAIT
SETMAR
HsMar1, METNASE, Mar1
SET domain and mariner transposase fusion gene
GO Process (15)
GO Function (10)
GO Component (3)
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IDA, IMP]
- DNA double-strand break processing [IDA]
- DNA integration [IMP]
- cell proliferation [IMP]
- double-strand break repair via nonhomologous end joining [IDA, IMP]
- histone H3-K36 dimethylation [IMP]
- histone H3-K36 methylation [IDA, IMP]
- histone H3-K4 methylation [IDA]
- mitotic DNA integrity checkpoint [IMP]
- negative regulation of cell cycle arrest [IMP]
- negative regulation of chromosome organization [IDA]
- nucleic acid phosphodiester bond hydrolysis [IMP]
- positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity [IDA, IMP]
- positive regulation of double-strand break repair via nonhomologous end joining [IDA]
- replication fork processing [IMP]
Gene Ontology Molecular Function- DNA topoisomerase binding [IPI]
- double-stranded DNA binding [IMP]
- endonuclease activity [IDA, IMP]
- histone methyltransferase activity (H3-K36 specific) [IDA]
- histone methyltransferase activity (H3-K4 specific) [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- single-stranded DNA binding [IMP]
- single-stranded DNA endodeoxyribonuclease activity [IMP]
- structure-specific DNA binding [IDA, TAS]
- DNA topoisomerase binding [IPI]
- double-stranded DNA binding [IMP]
- endonuclease activity [IDA, IMP]
- histone methyltransferase activity (H3-K36 specific) [IDA]
- histone methyltransferase activity (H3-K4 specific) [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- single-stranded DNA binding [IMP]
- single-stranded DNA endodeoxyribonuclease activity [IMP]
- structure-specific DNA binding [IDA, TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
TCEB2
ELOB, SIII
transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
GO Process (8)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- cellular response to hypoxia [TAS]
- gene expression [TAS]
- positive regulation of viral transcription [TAS]
- protein complex assembly [TAS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription from RNA polymerase II promoter [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]
Quantitative Score
- 17.35870636 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Curated By
- BioGRID